Characterization of cotton (<i style="">Gossypium hirsutum</i>) germplasm by ISSR, RAPD markers and agronomic values

Abstract

Using ISSR, RAPD markers and agronomic values, the characterization of 25 cotton (<i style="">G. hirsutum</i>) germplasms was carried out. 45 ISSR primers and 40 RAPD primers were used to amplify the germplasms. 19 scorable ISSR primers generated 90 markers while 21 reproducible RAPD primers generated 150 markers; of which, 49 markers from ISSR and 76 markers from RAPD were scored as polymorphic. Dendrograms were developed for ISSR and RAPD analysis by using NTSYS-pc software. Dendrogram of ISSR analysis showed 3 clusters while that of RAPD showed 4. Six agronomic values were also used for the characterization of germplasms. Based on these agronomic values, non-hierarchical Euclidean cluster analysis was performed to group the 25 cotton germplasms into 6 clusters. Significant similarities were found in the clustering of ISSR and RAPD analysis, however, correlations were not made between the clustering of ISSR, RAPD analysis and agronomic data analysis.