<smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> <i style="">Banana streak virus</i> from India and its detection by polymerase chain reaction </smarttagtype></smarttagtype>

Abstract

<i style="">Banana streak virus</i> (BSV) is an important pathogen of bananas and plantains (<i style="">Musa </i>spp.). The authors cloned and sequenced part of the genomes of two isolates of BSV from Kerala, BSV-K1 and BSV-K3. Both the clones contained a sequence covering a part of open reading frame III. The multiple sequence alignment of amino acids showed that both the isolates had a very high degree of identity with each other and clustered with Nigerian isolates of BSV (BSV-Onne) but not with Australian isolate of BSV (BSV-Mys). The relationship of these two isolates with other badnaviruses and<i style=""> Rice tungro bacilliform virus </i>was also analysed. BSV was detected by PCR amplification in samples, which were symptomatic but negative in electron microscopy.