<i style="">In vitro</i> propagation of <i style="">Pterocarpus marsupium</i> Roxb.: An endangered medicinal tree

Abstract

Nodal segments of <i style="">Pterocarpus marsupium</i> Roxb. were inoculated on seven different media compositions, viz. MS, B5 and White’s without growth hormones (MS_{00 ,} B5₀₀and WH₀₀), each supplemented with 3.0 mg l^-1 BAP and 0.5 mg l^-1 NAA (MSBN, B5BN, WHBN) and MS media supplemented with 0.2 mg l^-1 IBA (MSIB). Seed germination improved in all the media studied, however, MS combinations were the best (95-100%). Maximum number of shoot induction per explant was in MS₀₀ (3.25) followed by MSIB (2.26). Maximum nodes per shootlet were observed in medium MSIB (4.95), while shoot length was maximum in MSIB (2.92 cm) followed by MS₀₀ (2.41). Regenerated plants were acclimatized and successfully transferred under field conditions.