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http://nopr.niscpr.res.in/handle/123456789/5879| Title: | Selection of transformants of Escherichia coli containing cellulase gene from Ruminococcus albus isolated from rumen of crossbred steers |
| Authors: | Chandrasekharaiah, M Thulasi, A Sampath, K T |
| Keywords: | Ruminococcus albus;cellulase genes;transformants;Escherichia coli;rumen |
| Issue Date: | Jul-2004 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 C 12 N 15/10, 15/11 |
| Abstract: | Ruminococcus albus, considered best fibrolytic bacterium was isolated and characterized from the rumen of crossbred steers. It was found as wrinkled white colonies, slightly elevated with a slightly undulated margin, no surface spreading with the absence of liquefaction and a zone of hydrolysis. The cells were gram positive single cocci or diplococci. R. albus DNA was a high molecular weight DNA and it had just moved out of the well during electrophoresis. A genomic library of the Hind III fragments of R. albus DNA in pBR322 was constructed in Escherichia coli. Four clones were obtained with cellulase activity. |
| Page(s): | 431-434 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.03(3) [July 2004] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 3(3) 431-434.pdf | 62.74 kB | Adobe PDF | View/Open |
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