Effects of genotype and culture medium on <i style="">in vitro</i> androgenesis in soybean (<i style="">Glycine</i> <i style="">max</i> Merr.)

Abstract

Anthers of ten genotypes of <i style="">Glycine max</i> were cultured on four fortified B₅ media supplemented with different levels of growth hormones, viz. B₅ DBIG (2.0 mg l^-1 2,4-D+0.5 mg l^-1 IBA+100.0 mg l^-1 <i style="">myo</i>-Inositol+360.0 mg l^-1 L-glutamine), B₅ DB (2.0 mg l^-1 2,4-D+0.5 mg l^-1 BA), B₅DK (2.0 mg l^-1 2,4-D+0.5 mg l^-1 Kinetin) and B₅BKN (0.5 mg l^-1 BA+0.5 mg l^-1 Kinetin+1.0 mg l^-1 NAA). All the media were supplemented with 90.0 g l^-1 sucrose and 7.0 g l^-1 agar. Significant differences in the response of genotypes, culture medium and genotype x medium interactions were observed for callus initiation, formation of morphogenic calli and plantlet regeneration. Genotype JS 90-41was found superior for <i style="">in vitro</i> androgenesis. Culture medium B₅DBIG exhibited higher response for androgenic callus formation and haploid plant regeneration.