Establishment of an embryogenic suspension culture of <i style="">Pinus kesiya</i> (Khasi pine) from various explants

Abstract

<smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> The embryogenic cultures were obtained from mature zygotic embryos (79.6%) and apical dome sections (92.6%) (pretreated with 0.4% activated charcoal at 4^oC for 24 hrs) on mMS and ½DCR media, respectively containing 5 mg l^-1 2,4-D, NAA in combination along with 1 mg l^-1 BAP, while, from secondary needles (88.6%) it was on MS medium containing 3 mg l^-1 each of 2,4-D and NAA in conjunction with 1 mg l^-1 BAP. Using 150-200 mg embryogenic tissues per 15 ml medium at 120 rpm raised embryogenic suspension cultures. But the embryogenic cultures from apical dome sections were cold treated at 4^oC for 24 hrs before transferring to suspension culture. The cultures were subcultured at 6-7 days interval and were diluted at 1:4 ratio. The embryonal suspensor masses developed from the resulting elongated single cells within 3-4 passages in respective basal medium with 1/10^th growth regulators of initiation medium. The proembryonal head formed in growth regulators free medium at 100 rpm. The embryonal head formed in basal medium containing 4% sucrose and 4 mg l ^-1 ABA in combination. </smarttagtype></smarttagtype>