Genetic characterisation of Jaisalmeri camel using microsatellite markers

Abstract

Six New World Camelidae microsatellite primer pairs were used to investigate the genetic polymorphism in Jaisalmeri camel. Polymerase chain reactions were carried out for 30 unrelated camels of Jaisalmeri breed. The amplification products were resolved in 6% (denaturing) urea PAGE and stained with silver nitrate. All the 6 microsatellite primer pairs were found polymorphic in Jaisalmeri camel. The number of alleles ranged from 2 to 5. The expected heterozygosity ranged from 0.32 to 0.651 and the polymorphic information content ranged from 0.268 to 0.588. The results indicated the utility of these microsatellite loci for studying genetic polymorphism in dromedary breeds.