Plant regeneration from callus cultures in <i style="">Suaeda nudiflora </i>(Wild.) Moq.

Abstract

<i style="">Suaeda nudiflora </i>plants, showing relatively high salt tolerance, were used for micropropagation and generation of plantlets from callus cultures. Induction of somaclonal variability in the existing germplasm was another aim. Callus was initiated from young stem on MS medium supplemented with different concentrations of BAP and 2,4-D. Organogenesis was achieved by transferring callus on MS medium supplemented with BAP, KN and adenine. Well-grown shoots rooted easily on MS-half supplemented with IBA, IPA and NAA and 80-90% rooting was achieved. Plantlets got hardened by keeping them in hardening unit for few days. Hardened plants established very well in the nursery. Though originated from callus, these plants did not show any morphological variations and were similar to their parent donor plant.