Molecular detection and phylogenetic analysis of lumpy skin disease virus from outbreak in Madhya Pradesh, India 2020

Abstract

Lumpy skin disease (LSD) is an emerging, economically important infectious viral disease of cattle caused by Capripoxvirus. An outbreak of LSD has been reported in various states including Madhya Pradesh, India. This study was undertaken to do molecular characterization of the lumpy skin disease virus (LSDV) and to study its phylogenetic relationship with the sequences available in National Centre for Biotechnology Information (NCBI) database. A total of 6 blood samples and 2 tissue skin nodule samples were collected from cattle (aged from 6 months to 6 years) biopsies from Bamhnoda & Sagda Jhapani village of Jabalpur District of Madhya Pradesh, India and were screened for LSDV by PCR. The samples were subjected for amplification of P32 and fusion genes. Two tissue and three blood samples were positive for P32 gene. Partial P32 and fusion genes were amplified and sequenced and subjected for phylogenetic analysis by MEGA6. The phylogenetic analysis based on partial P32 gene and fusion gene showed close proximity with isolates from Egypt, Saudi Arabia and China.