“In vivo” free radical scavenging efficacy of beebread against biochemical alterations induced by Salmonella enterica serovar Typhimurium

Abstract

Salmonella typhimurium induced hepatic and renal toxicity was investigated using standard laboratory techniques in mice. BALB/c mice were divided into ten groups (8 mice per group). Group (Gp) 1 served as normal, and were administrated with normal saline orally, and Gp2 served as infected, and were injected bacteria (2×104 CFU/mL) only, while Gp3, 5, 7 and, 9 were administrated beebread (250 mg/kg body weight) of Helianthus annus, Brassica campestris, Zea mays and vitamin C respectively for 21 days without Salmonella infection, and Gp 4, 6, 8 and, 10 were administrated beebread (250 mg/kg bw) of Helianthus annus, Brassica campestris, Zea mays and vitamin C respectively for 21 days with a bacterial infection. Serum activities of hepatic and renal enzymes were analysed. A significant increase was observed in biochemical enzymes in Salmonella infected group on the 5th day but after the administration of beebread of different crops, relief was observed to be near normal. This alleviation was more with beebread of Helianthus annus. Furthermore, administration of beebread without bacteria did not show any negative effects in mice. Thus, the results indicate that aqueous extract of beebread of different crops is safe and can be exploited in healthcare delivery systems.