Pongamol from the seeds of Tephrosia purpurea exert anti-cancer activity against cervical cancer cell line

Abstract

Tephrosia purpurea belongs to the family Fabaceae, is used for the treatment of inflammation, diabetes, cancer, chronic fever, boils, gingivitis etc. In the present study, three compounds (TP-1, TP-3 and TP-5) were isolated from ethyl acetate extract of the seeds of T. purpurea (EETP). Quantity of TP-3 (6.05%) in EETP was determined by HPLC. In-vitro anti-cancer activity of EETP and isolated compound TP-3 on SiHa cells as well as PBMCs (peripheral blood mononuclear cells) were evaluated by MTT (3-(4,5- di methyl thiazol -2-yl)-2,5-di phenyl tetrazolium bromide) assay. On the basis of spectroscopic analysis and physical properties, structure of isolated compounds TP-1, TP-3 and TP-5 were characterized as teclenone, pongamol and β-sitosterol respectively. Isolated compound TP-3 showed cytotoxicity against SiHa cells with the IC50 33.06 μgmL-1. TP-3 showed significant cytotoxicity at the concentration range from 20-50 μgmL-1 as compared to control. Maximum cytotoxicity (72.75%) was observed at the concentration of 50 μgmL-1. TP-3 showed non-significant cytotoxicity against PBMCs cells at all the concentrations except at 50 μgmL-1 (cytotoxicity 24.19%). EETP showed significant cytotoxicity (IC50 value 113.63 μgmL-1) against SiHa cells at the concentration range from 75-150 μgmL-1 as compared to control. Maximum cytotoxicity (75.34%) was observed at the concentration of 150 μgmL-1.