Effect of C-terminal 44 amino acids deletion on activity of <i style="">Haemophilus influenzae</i> UvrA protein

Abstract

UV-sensitive mutant strain of <i style="">Haemophilus influenzae</i> Rd MBH3, is 20 times more sensitive to UV irradiation than the wild type strain. The mutation responsible for increased UV sensitivity of the strain was identified as G→A transition predicting synthesis of truncated UvrAΔC44 protein (Balsara & Joshi)¹². Recombinant UvrAΔC44 protein was purified for the first time under denaturing conditions. The molecular weight of the recombinant protein was estimated as ~100 kDa. Recombinant UvrAΔC44 protein was found to be less efficient in its ATPase and DNA binding activity as compared to the wild type protein. Recombinant plasmid carrying <i style="">uvr</i>AΔC44 gene could partially complement the UvrA deficiency in <i style="">E. coli</i> UvrA mutant.