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Title: In vitro antioxidant activity of Diospyros malabarica Kostel bark
Authors: Mondal, Susanta Kumar
Chakraborty, Goutam
Gupta, M
Mazumder, U K
Keywords: Antioxidant;Diospyros malabarica;Free radicals;Phenolic compound;Reductive ability
Issue Date: Jan-2006
Publisher: CSIR
Abstract: Antioxidant activity of defatted methanol extract of D. malabarica bark was studied for its free radical scavenging property on different in vitro models e.g. 1,1-diphenyl-2-picryl hydrazyl (DPPH), nitric oxide, superoxide, hydroxyl radical and lipid peroxide radical model. The extract showed good dose-dependent free radical scavenging property in all the models except in hydroxyl radical inhibition assay. IC50 values were found to be 9.16, 13.21, 25.27 and 17.33 µg/ml respectively in DPPH, nitric oxide, superoxide and lipid peroxidation inhibition assays. In hydroxyl radical inhibition assay 1000 µg/ml extract showed only 10% inhibition with respect to the control. Measurement of total phenolic compounds by Folin-Ciocalteu’s phenol reagent indicated that 1mg of the extract contained 120.7µg equivalent of pyrocatechol. The results indicate that the antioxidant property of the extract may be due to the high content of phenolic compounds. However, the underlying mechanism may not involve hydroxyl radical scavenging property.
Page(s): 39-44
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(01) [January 2006]

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