<i style="">In vitro</i> clonal propagation of <i style="">Chlorophytum borivilianum</i> Sant. et Fernand., a rare medicinal herb from immature floral buds along with inflorescence axis

Abstract

A novel method of shoot regeneration from immature floral buds along with inflorescence axis in <i style="">C. borivilianum</i>, a rare medicinal herb is described. Using this explant, axenic cultures were established with very less contamination (10%). MS medium with 2 mg l^-1 kinetin and 0.1 mg l^-1 2, 4-dichlorophenoxyacetic acid proved to be the best for multiple shoot induction. Maximum number (35) of shoot production was achieved in MS medium with 2 mg l^-1 benzylaminopurine. Rooting of shoots (86.7%) with maximum fasciculated roots (5) occurred on Knops medium containing iron and vitamins of MS medium with 2 mg l^-1 indole-3-butyric acid and 0.1% activated charcoal. Plant survival was 80% in four weeks after their removal from <i style="">in vitro</i> conditions. Per explant 34 hardened plants generated within 50 weeks. This protocol can be useful for large-scale clonal multiplication from immature floral buds with inflorescence axis and successfully used for germplasm conservation of this rare medicinal herb without destroying the mother plant.