Study of Protective Effect of Curcumin and Quercetin Against Subacute Toxicity of Fipronil in Rats

Abstract

Fipronil is belonged to phenyl pyrazole class of pesticides, used in the veterinary, public health, and agricultural fields to control a variety of pests. Numerous researchers have looked into the possibility that long-term exposure to fipronil causes liver and renal disease. It has been shown that curcumin and quercetin provide protection against the toxicity caused by numerous pesticides. The purpose of our study was to investigate the possible preventive role of quercetin and curcumin in hepatic and renal damage caused by fipronil. The rats were divided into five groups, with six rats in each group. Group I was not given any medication and treated as control, while rats of treatment groups were orally administered with the fipronil at dose 10 mg∙kg−1b.wt to group I, fipronil and curcumin (10 mg∙kg−1b.wt +100 mg∙kg−1b.wt) to group III, fipronil and quercetin (10 mg∙kg−1b.wt +100 mg∙kg−1b.wt ) to group IV and combination of fipronil, curcumin and quercetin (10 mg∙kg−1b.wt +100 mg∙kg−1b.wt +100 mg∙kg−1b.wt ) to group V. After exposure, biochemical markers were measured on days 0, 14, and 28th day of study period. Body weight of rats significantly decreased throughout the course of 28 days of exposure to fipronil. Hepatic and renal damage caused by fipronil was indicated by significant increase in the serum levels of AST, ALT, ALP, LDH, BUN, and creatinine, which were recorded at different intervals during the study period. Nevertheless, the negative effects of fipronil were significantly reduced by supplementing with curcumin and quercetin, both separately and together. This was demonstrated by a considerable drop in the raised level of serum biochemical markers. The current study concludes that quercetin and curcumin may function as effective protective agents against toxicity generated by fipronil.