Synthesis and characterization of (E)-N-carbamimidoyl -4 and (E)-4- benzenesulfonamides; biological study, DFT, molecular docking, and ADMET predictions

Abstract

Sulphonamide Schiff bases (L1 and L2) containing imidazole nuclei have been synthesized and evaluated for their antimicrobial and antioxidant activity. Sulfaguanidine and sulfamerazine have been condensed with 4-methyl-5- imidazolecarboxalehyde to obtain ligands L1 and L2, respectively. The compounds have been characterized by FT-IR, 1H and 13C NMR, UV-Vis, CHNS, and MALDI-TOF mass spectral data. The antimicrobial activity of the sulphonamidederived Schiff bases have been conducted using agar well diffusion against S. aureus, B. substilis, E. Coli, Salmonella spp., and Candida spp. Similarly, the free radicals scavenging activity of the compounds has been evaluated at 20 – 100 μg/mL using DPPH (1,1’-diphenyl-2-picryl-hydrazil), nitric oxide, and hydrogen peroxide antioxidant assays. Both compounds exhibited moderate activity against Salmonella spp. However, L1 exhibits higher radical scavenging ability than L2 against NO free radicals at low to high concentrations with IC50 values of 84.50 and 101.59 μg/mL for L1 and L2, respectively. Ligand L2 is however, more active than L1 against H2O2 free radicals at low concentrations (20 – 60 μg/mL). The optimized geometries of the compounds have been docked at the active sites of cytochrome oxidase, myeloperoxidase, NADPH oxidase, xanthine oxidase, dihydropteroate synthase (DHPS), and dihydrofolate reductase (DHFR) proteins.