Vermicompost Derived Pseudomonas Plecoglossicida Strain Efficient of Growth Promoting and Managing of Root Rot Disease in Pea Plants (Pisum Sativum)

Abstract

The present evaluation is to characterise and assess the selected novel vermicompost-based Plant Growth–Promoting (PGP) bacteria on pea crops. Vermicompost tea was prepared using the production assembly of two plastic sieve containers and a storage bin. Among the five bacterial isolates from vermicompost tea, a single prominent isolate was found optimistic for many of the PGPR traits like nitrogen fixation, indole acetic acid production, phosphate solubilization, plant defence enzymes, anti–fungal activity and siderophore secretion. The bacterization of pea seeds with vermicompost tea and N3 bacterial culture indicated an increase in germination, vigour index and fresh seed weight, when compared to the control. The isolate showed significant growth and exhibited a variety of plant growth- promoting traits on pea crops, making it a suitable inoculant. The selected bacterium showed antifungal activity against Fusarium oxysporium and prominent expression of β–1,3glucanase, phenylalanine ammonia–lyase, peroxidase and chitinase defense proteins. The pot study of the pea plants treated with the vermicompost and N3 isolate on the 5th, 10th, and 15th day showed the optimum root length, shoot length, and dry biomass weight with reference to the control. The isolate was identified as Pseudomonas plecoglossicida [Yank1] derived from the phenotypic and genotypic characteristics. The Yank1 strain received accession number MF153408 to deposition in GenBank. Thus, the current study proposes the beneficial effects of Pseudomonas plecoglossicida isolated from vermicompost tea for the first time as PGPR on pea plant through collective action mode.