One pot synthesis of benzimidazole derivatives with antimicrobial evaluation

Abstract

This study evaluates the antibacterial and antifungal activity of several novel synthesized title benzimidazoles S2-e, S2-g, S2-q, S2-o by coupling 3-amino-N,N-dimethyl-4-(methylamino)benzamide) S2-c with methoxy amine, 2-(((2,6- dimethylphenyl)glycyl)oxy)acetic acid S2-f, (2-methylbenzaldehyde) S2-p and ((4-(5-methyl-1,2,4-oxadiazol-3- yl)phenyl)glycine) S2-n in presence of carbonyl diimidazole compounds against various bacterial and fungal strains. The compounds have been synthesized using a cost-effective and less hazardous route. 1H and 13C NMR, and mass spectroscopy have been used to characterize the synthesized compounds. The Minimal Inhibition Concentration (MIC) values for the antibacterial and antifungal assays have been determined for each compound and compared with standard drugs such as Gentamycin, Ampicillin, Nystatin, and Griseofulvin. The results show that compound S2-f demonstrates notable antibacterial and antifungal properties, making it a candidate for further medicinal development. Molecular docking of S2-e has been performed on the binding site of Candida albicans CYP 51 (PDB id: 5TZ1).