Enzymatic hydrolysis of chemically pretreated rice straw by two indigenous fungal strains: a comparative study

Abstract

<smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> This study presents pretreatment of rice straw using ethylene diamine, (EDA 28%), hydrochloric acid (HCl 5%) and sodium hydroxide (NaOH 2%) and on-site enzyme production from two <i>Aspergillus </i>species. Pretreated rice straw was enzymatically hydrolyzed with crude cellulase from <i>A. niger </i>and <i>A. heteromorphus</i>. Enzymatic hydrolysis conditions (pH and temperature) were optimized by RSM model. Maximum activity was found by <i>A. niger </i>(FPase, 7.4 IU/g; CMCase, 97 IU/g) and <i>A. heteromorphus </i>(FPase, 9.5 IU/g; CMCase 125 IU/g) in 72 h with EDA pretreated rice straw followed by NaOH and HCl pretreatment. </smarttagtype></smarttagtype>