Plant regeneration from semi-mature zygotic embryos of <i style="">Dalbergia sissoo </i>Roxb.

Abstract

Plant regeneration from callus cultures derived from semi-mature zygotic embryos of <i style="">Dalbergia sissoo </i>Roxb. was achieved. Callus formation occurred on Murashige and Skoog (1962) medium supplemented with 2.26-13.57 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> 2,4-D in combination with 0.46 and 1.16 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> Kn. Maximum response for callus formation was 78.3% on MS medium containing 9.04 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> 2,4-D and 1.16 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> Kn. Shoot regeneration occurred when calli clumps were transferred to MS medium enriched with 2.22-13.32 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> BAP and 1.34 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> NAA. Maximum response (45%) for shoot regeneration was achieved when calli clumps were transferred to MS medium supplemented with 8.88 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> BAP and 1.34 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> NAA. Average number of shoots per callus clump was 7.5 after 15 weeks of culture. <i style="">In vitro</i> regenerated shoots were rooted on ½ MS medium containing 1.23 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> IBA. Rooted plantlets transferred to pots containing autoclaved peat moss, soil, and compost mixture (1:1:1) were successfully established in pots.