Production and effect of killer toxin by <i style="">Saccharomyces cerevisiae</i> and <i style="">Pichia kluyveri</i> on sensitive yeasts and fungal pathogens

Abstract

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Killer yeasts from flowers of Indian medicinal plants were isolated and the effect of their killer toxin was determined on sensitive yeast cells as well as fungal pathogens. The toxin of <i style="">Saccharomyces cerevisiae</i> and <i style="">Pichia kluyveri</i> inhibited <i style="">Dekkera anomala</i> accumulating methylene blue cells on Yeast Extract Peptone Dextrose agar (<i style="">p</i>H 4.2) at 21°C. There was no inhibition of growth or competition between the yeast cells in the mixed population of <i style="">S. cerevisiae</i> isolated from <i style="">Acalypha indica</i>. <i style="">S. cerevisiae</i> and <i style="">P. kluyveri</i> were found to tolerate 50% and 40% glucose, while <i style="">D. anomala</i> tolerated 40% glucose. Both <i style="">S. cerevisiae</i> and <i style="">P. kluyveri</i> did not inhibit the growth of <i style="">Aspergillus niger</i>, C<i style="">andida albicans </i>and <i style="">Fusarium </i>spp.

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