PCR-RFLP based genotyping of cattle using DNA extracted from hair samples

Abstract

A simple method of genotyping of farm animals using DNA extracted from hair samples is described. Hair samples of 35 F <img src='/image/spc_char/cross.gif' border=0> H (Holstein Friesian <img src='/image/spc_char/cross.gif' border=0> Hariana) crossbred cattle were processed for isolation of genomic DNA. These DNA samples were used for genotyping of insulin-like growth factor binding protein 3 (IGFBP 3) gene using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Thus, hair samples could be an easy and reliable source of DNA for genotyping of farm animals or any other molecular genetic research work where DNA is needed as an experimental material for the study.