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http://nopr.niscpr.res.in/handle/123456789/7755| Title: | Somatic embryogenesis and plant regeneration in Soybean [Glycine max (L.) Merr.] |
| Authors: | Kumari, B D Ranjitha Settu, A Sujatha, G |
| Keywords: | embryonic axes;callus;somatic embryogenesis;plant regeneration;Glycine max |
| Issue Date: | Apr-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00 |
| Abstract: | Establishment of efficient protocol for the induction of somatic embryogenesis and plant regeneration from embryonic axes explants was achieved in Glycine max (L.) Merr. Highest frequency (92.9%) of embryogenic callus formation was obtained from embryonic axes explants of cv. CO-1 on MS media containing 2,4-Dichlorophenoxy acetic acid (2,4-D) and 6-benzyl adenine (BA). Fast growing, yellowish nodular callus lines containing somatic embryos were established on initiation medium supplemented with 180.8 µM 2,4-D and 2.22 µM BA, respectively. Embryo development and maturation was achieved on MS medium supplemented with 2.26 µM 2,4-D and 8.88 µM BA. The well formed embryos germinated into complete plantlets on MS medium containing 8.88 µM BA and 1.34 µM NAA (a-Naphthalene acetic acid). The regenerated plants were first transferred to plastic cups and later into pots for maturation. Regenerated plants did not exhibit observable morphological alterations. |
| Page(s): | 243-245 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.05(2) [April 2006] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 5(2) 243-245.pdf | 519.2 kB | Adobe PDF | View/Open |
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