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http://nopr.niscpr.res.in/handle/123456789/8572| Title: | A new method for spectrophotometric determination of colchicoside |
| Authors: | Narayana, B Divya, N S |
| Keywords: | Azure B (AB);Ceric ammonium sulphate (CAS);Colchicoside (COLSID);Safranin O (SO) |
| Issue Date: | May-2010 |
| Publisher: | CSIR |
| Abstract: | A facile spectrophotometric method is proposed for determination of Colchicoside (COLSID) in bulk and in dosage forms using ceric ammonium sulphate (CAS). Method involves addition of a known excess of CAS to COLSID and determination of residual CAS with a fixed amount of either Azure B (AB), measuring absorbance at 633nm or Safranin O (SO), measuring absorbance at 519 nm. Calibration graphs are linear over 25.00-50.00 μgml-1, 22.00-38.00 μgml-1 and apparent molar absorptivity is calculated to be 2.28 x 104 l mol-1 cm-1 and 2.77 x 104 l mol-1 cm-1 for CAS-AB and CAS-SO respectively. Method is successfully applied to assay of COLSID in pharmaceutical formulations. |
| Page(s): | 368-372 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Appears in Collections: | JSIR Vol.69(05) [May 2010] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| JSIR 69(5) 368-372.pdf | 125.33 kB | Adobe PDF | View/Open |
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