Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/9078| Title: | Isolation of genomic DNA from medicinal plants without liquid nitrogen |
| Authors: | Sharma, Pratibha Joshi, Neha Sharma, Anubhuti |
| Keywords: | CTAB;DNA isolation;Liquid nitrogen;PCR;RAPD |
| Issue Date: | Jun-2010 |
| Publisher: | CSIR |
| Abstract: | Genomic DNA was extracted from eight medicinal plants using the present DNA extraction protocols (CTAB extraction method) with some modifications. Leaves were fixed in different fixing solutions containing absolute alcohol (99.99%), chloroform and EDTA, but without liquid nitrogen. DNA quality and quantity obtained were comparable to those isolated with liquid nitrogen, as the λ260/λ280 ratio with liquid nitrogen was in range 1.3-1.7 and with other fixing solutions it was 1.1-1.5. Absolute alcohol showed best results as fixing solution. Good quality of DNA was isolated without using liquid nitrogen from different medicinal plant species. DNA isolated by this method was suitable for various molecular biology applications. |
| Page(s): | 610-614 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.48(06) [June 2010] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 48(6) 610-614.pdf | 603.7 kB | Adobe PDF | View/Open |
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