Uterine tonic activity of Siddha Kayakarpam Amuri in rats

Abstract

Siddha Kayakarpam Amuri was evaluated for its estrogenic activity using in-vivo and in-vitro experimental models. Estrogenic effect was studied in normal and ovariectomized rats. Amuri was administered for a period of 21 days. The parameters studied in both in-vivo models include changes in uterine weight, and histometric changes of uterus. The effect was also studied on normal and regular estrous cycle. In-vitro studies with Amuri on non-primed, estrogen-primed and Amuri pretreated uterus were carried out to find whether it possess any oxytocin like activity. Administration of Amuri in normal rats significantly increased the uterine weight. Amuri treatment in ovariectomized rats did not show any change in uterine weight. The rats from both control and treated groups showed normal estrous cycle and produced significant contractile response on the non-primed, estrogen-primed and Amuri pretreated rat uterus when exposed in-vitro.