Enzymatic saccharification of cellulosic waste by cellulase system of <i style="">Cellulomonas uda</i> immobilized on tri(4-formyl phenoxy) cyanurate

Abstract

Cellulose is one of the most abundant non-degradable organic compound on earth. Near about half of the municipal and agricultural solid wastes contain cellulose or their derivatives. Saccharification or enzymatic hydrolysis of cellulosic wastes liberates glucose. In the present work, cellulase from <i style="">Cellulomonas</i> <i style="">uda</i> was extracted, partially purified by dialysis and immobilized on an organic support namely tri(4-formyl phenoxy) cyanurate. Percentage saccharification of seven different cellulosic waste materials was studied using native and immobilized cellulase systems. Maximum saccharification for both native and immobilized cellulase was observed for sawmill dust or wood dust (4.9 and 2.4% respectively) as compared to other cellulosic waste substrates.