Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/9832| Title: | Micropropagation of Tribulus terrestris Linn |
| Authors: | Raghu, A V Martin, Gerald Balachandran, Indira Mohanan, K V |
| Keywords: | Direct shoot regeneration;Medicinal plant;Micropropagation;Nodal explants;Tissue culture;Tribulus terrestris;Woody Plant Medium |
| Issue Date: | Jun-2010 |
| Publisher: | CSIR |
| IPC Code: | Int. cl.8 ¾ A61K 36/00, A01G 7/00, A01G 31/00 |
| Abstract: | A simple and efficient method for high frequency direct shoot regeneration from nodal explants of Tribulus terrestris Linn. (Family ¾ Zygophyllaceae), a medicinally important plant is described in this paper. Woody Plant Medium (WPM) supplemented with 4.0 mg/l BA was found to be most effective in inducing bud break, growth and also in initiating multiple shoot proliferation at the rate of six to seven micro-shoots per nodal explant after four weeks of culture. A high frequency multiplication rate was established by repeated sub-culturing. Excised shoots were rooted on MS basal medium without any growth regulators and got established after a hardening process with 80% survival. Micropropagated plants grew well under field conditions, attained maturity and flowered. No phenotypical differences were observed among regenerated plants. |
| Page(s): | 232-235 |
| Appears in Collections: | IJNPR Vol.1(2) [June 2010] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJNPR 1(2) 232-235.pdf | 489.94 kB | Adobe PDF | View/Open |
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