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Title: Establishment of a rapid multiplication protocol of Coleus forskohlii Briq. and in vitro conservation by reduced growth
Authors: Dube, Pratibha
Gangopadhyay, Moumita
Dewanjee, Saikat
Ali, M Nasim
Keywords: Coleus forskohlii;in vitro conservation;Mannitol;Micropropagation;Slow growth
Issue Date: Apr-2011
Publisher: NISCAIR-CSIR, India
Abstract: Coleus forskohlli Briq., an endangered medicinal plant, has been successfully micropropagated under in vitro conditions. Best adventitious shoots were developed in solid Murashige and Skoog’s medium supplemented with 1.5 mg L-l 6-benzylaminopurine, while best rhizogenesis was achieved without growth regulator. The second event of success was in vitro conservation of the species by slow growth technique employing osmotic regulators (sorbitol and mannitol). 3 M mannitol showed best performance for reducing growth. This study elucidated an efficient method of ex situ conservation of germplasm and sustainable management of this medicinally important plant.
Page(s): 228-231
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.10(2) [April 2011]

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