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http://nopr.niscpr.res.in/handle/123456789/12964| Title: | Assessing role of Cra in regulation of prpB and yahA genes of Escherichia coli in vivo using lacZ transcriptional fusions |
| Authors: | Ponmani, Thangaraj Munavar, M Hussain |
| Keywords: | Cra;lacZ;prpB;yahA;Transcriptional fusion |
| Issue Date: | Nov-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This study presents in vivo role of Cra (Catabolite repressor activator) protein in regulation of two selected genes (prpB & yahA) of Escherichia coli. Gene prpB bears Cra binding site and yahA region binds to Cra in vitro. Therefore, in pBR322 background, pprpB-lacZ and pyahA-lacZ transcriptional fusions were generated and studied expression of b-galactosidase from both fusions in cra+ and cra¯ genetic backgrounds of E. coli. Results that both the promoters could possibly be up-regulated by Cra in vivo in E. coli were discussed in relation to transcription control by accessory transcription factor(s). |
| Page(s): | 938-944 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Appears in Collections: | JSIR Vol.70(11) [November 2011] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| JSIR 70(11) 938-944.pdf | 303.05 kB | Adobe PDF | View/Open |
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