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http://nopr.niscpr.res.in/handle/123456789/13255| Title: | Preparation, crystallization and preliminary X-ray crystallographic analysis of OXA-23, a carbapenemase conferring widespread antibiotic resistance |
| Authors: | Lee, Jung Hun Sohn, Seung Ghyu Jung, Ha Il An, Young Jun Lee, Jae Jin Kang, Lin-Woo Lee, Sang Hee |
| Keywords: | Antibiotic resistance;Imipenem;Crystal;OXA-23;Carbapenemase;Acinetobacter baumannii |
| Issue Date: | Dec-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | OXA-23, a class D carbapenemase that confers widespread antibiotic resistance hydrolyzes the β-lactam rings of β-lactam antibiotics, presenting an enormous challenge to infection control, particularly in the eradication of pathogenic bacteria such as Acinetobacter baumannii, one of six top-priority dangerous pathogens. Thus, the enzyme is a potential target for developing antimicrobial agents against pathogens producing carbapenemases. In this study, OXA-23 was purified and crystallized at 298 K and X-ray diffraction data from OXA-23 crystal were collected at 2.03 Å resolution using synchrotron radiation. The crystal of OXA-23 belonged to space group P41 with unit cell parameters a = 82.47, b = 82.47 and c = 172.01 Å. Analysis of the packing density showed that the asymmetric unit probably contained two molecules with a solvent content of 73.64%. |
| Page(s): | 395-398 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Appears in Collections: | IJBB Vol.48(6) [December 2011] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBB 48(6) 395-398.pdf | 405.99 kB | Adobe PDF | View/Open |
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