Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/14525| Title: | A new method for determination of ascorbic acid in fruit juices, pharmaceuticals and biological samples |
| Authors: | Janghel, Etesh K Sar, Santosh Pervez, Y |
| Keywords: | Ascorbic acid;Auramine O dyes;Biological Samples;Pharmaceuticals;Spectrophtotometry |
| Issue Date: | Aug-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This study presents a new, selective and accurate indirect spectrophotometric method for determination of L-ascorbic acid in fruit juices, pharmaceuticals and biological samples. Beer’s law is obeyed by ascorbic acid (conc. range, 0.8-6.3 mg) per 25 ml of final solution (0.032-0.252 ppm). The method showed: apparent molar absorptivity, 1.56 × 105 l mol-1cm-1; Sandell’s sensitivity, 0.0024 mg cm-2; limit of detection, 0.0086 mg ml-1; and limit of quantitation, 0.01 mg ml-1. It was satisfactorily applied for determination of ascorbic acid in fruit juices, pharmaceuticals and biological samples. Reliability of method was established by parallel determination against Leuco crystal violet and Rhodamine – B method. |
| Page(s): | 549-555 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Appears in Collections: | JSIR Vol.71(08) [August 2012] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| JSIR 71(8) 549-555.pdf | 87.68 kB | Adobe PDF | View/Open |
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