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http://nopr.niscpr.res.in/handle/123456789/15272| Title: | Resolution of a complex ionic mixture of an apparently homogenous protein preparation by preparative electrophoresis |
| Authors: | Dubey, Ashok K |
| Issue Date: | Aug-2002 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Preparations of recombinant envelope glycoprotein E2 of hepatitis C virus (r-HCV E2), found to be homogeneous by N-terminal amino acid sequencing and mass spectrometry, resolved into multiple ionic species (isoforms) when analysed by isoelectric focusing (IEF) gel electrophoresis in the pI range of 3-10. These isoforms possessed pI values in the range or 4.5-8.2. The major isoform with pI value of approximately 7. 1 was separated from the rest of them by employing a method developed on Gradiflow BF 200, a device based on preparative electrophoresis. This isoform was adjudged to be homogenous by IEF and by native polyacrylamide gel electrophoresis (PAGE). |
| Page(s): | 279-283 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Appears in Collections: | IJBB Vol.39(4) [August 2002] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBB 39(4) 279-283.pdf | 1 MB | Adobe PDF | View/Open |
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