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http://nopr.niscpr.res.in/handle/123456789/15393| Title: | Analysis of the activity of promoters from two photosynthesis-related gene psaF and petH of spinach in a monocot plant, rice |
| Authors: | Mohanty, Amitabh Grover, Monendra Chaudhury, Ashok Rizwan-ul-Haq, Quazi Sharma, Arun K Maheshwari, Satish C Tyagi, Akhilesh K |
| Issue Date: | Dec-2000 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The subunit III of phtosystem I and ferredoxin-NADP+ -oxidoreductase are encoded by nuclear genes, namely psaF and petH. The activity of their promoters from spinach has been evaluated in transgenic tobacco earlier. Evaluation of the activity of these Dicotyledoneae-specific promoters has been carried out in a monocot system (i.e. rice) by transient gene expression system, based on electroporation-mediated gene delivery into protoplasts from leaves and roots. It has been found that various promoter deletions show higher activity in leaf protoplasts and elements for quantitative response are widely distributed. Transgenic rice has also been produced with a petH promoter and gus reporter gene construct. Although petH promoter is a weak promoter in comparison to the 35S promoter, it expresses well in green tissues and could be useful for plant genetic engineering. |
| Page(s): | 447-452 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Appears in Collections: | IJBB Vol.37(6) [December 2000] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBB 37(6) 447-452.pdf | 1.3 MB | Adobe PDF | View/Open |
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