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http://nopr.niscpr.res.in/handle/123456789/15897| Title: | Synthesis, crystal structure and spectroscopic investigations on organic complex salt and its interaction with bovine serum albumin |
| Authors: | Meng, Fa-Yan Yu, Sheng-Rong Ma, Lin Lin, Cui-Wu |
| Keywords: | Organic complex salts;Chlorogenic acid;Fluorescence spectroscopy;Bovine serum albumin |
| Issue Date: | 5-Feb-2013 |
| Abstract: | A new organic complex salt based on chlorogenic acid with 1,10-phenanthroline has been synthesized and characterized. The complex adopts 1:1 stoichiometry in the solid state. The proton transfer occurs from the carboxyl oxygen to the aromatic nitrogen atom to form the salt. The Hphen molecule individually occupies the pseudo-tetragonum that is formed with chlorogenic acid. The self-assembly of the compound in solid state is caused by a hydrogen-bonding interaction. The interaction of the organic complex salt with bovine serum albumin molecules has been studied by fluorescence spectroscopy. The bimolecular quenching constant, apparent quenching constant and number of binding sites have been obtained by fluorescence quenching and the binding distance calculated according to Foster’s non-radioactive energy transfer theory. The corresponding thermodynamic parameters ΔH, ΔG and ΔS have also been calculated. The conformational changes of BSA have been observed from synchronous fluorescence spectra. |
| Page(s): | 192-199 |
| ISSN: | 0975-0975(Online); 0376-4710(Print) |
| Appears in Collections: | IJC-A Vol.52A(02) [February 2013] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJCA 52A(02) 192-199_Suppl Data.pdf | Supplementary data | 400.28 kB | Adobe PDF | View/Open |
| IJCA 52A(2) 192-199.pdf | Main article | 433.04 kB | Adobe PDF | View/Open |
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