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http://nopr.niscpr.res.in/handle/123456789/16993| Title: | In vitro mass multiplication of Ophiorrhiza mungo Linn. |
| Authors: | Jose, Binoy Satheeshkumar, K |
| Issue Date: | Jun-2004 |
| Publisher: | NISCAIR-CSIR, India |
| IPC Code: | Int Cl7 A01H4/00 |
| Abstract: | A protocol for in vitro mass multiplication of plants through seedling (shoot) cultures was established for Ophiorrhiza mungo. Maximum number of adventitious shoots per shoot culture (10.4 ± 1.72) was initiated on MS solid medium supplemented with BAP (2.22μM) after 3 weeks. Shoots were further multiplied (12.8 ± 2.8) through subculture of intact shoots and reculture of nodal segments of aseptic shoots (6.5±0.94) in MS solid medium containing BAP (0.89μM). Shoot elongation (1.27 ± 0.1 2 cm) was achieved in the medium containing GA3 (1.44μM) in two weeks. Rooting was favoured in basal agar medium supplemented with IBA (12.3μM) plus NAA ( 1.07μM). The plants were successfully established (100%) in the pots containing sand and top soil (1:1) mixture in a period of two weeks. |
| Page(s): | 639-642 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.42(06) [June 2004] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 42(6) 639-642.pdf | 842.57 kB | Adobe PDF | View/Open |
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