Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/19285| Title: | Regeneration of plantlets from nodal explant-derived callus cultures of Albizia lucida Benth. |
| Authors: | Saha, Dipnarayan Kumar, Rajendra Ramani, Ranganathan |
| Keywords: | Acclimatization;Albizia lucida;Callus-mediated regeneration;Genetic fidelity;Lac host plant micropropagation |
| Issue Date: | Apr-2013 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Albizia lucida Benth. is a forest tree known for timber, gum and tannin, and lac production. Micropropagation of A. lucida can potentially address large-scale production of genetically identical superior genotypes. In the present study, a callus-mediated regeneration protocol has been established from nodal explants of A. lucida for micropropagation. Murashige and Skoog’s (MS) medium supplemented with plant growth regulators like thidiazuron (TDZ; 0.5 µM) and 6-benzylaminopurine (BAP; 2.22 mM) were found efficient in callus induction and multiplication. From the multiplied greenish and compact organogenic calli, shoots were regenerated best on MS media consisting BAP (8.88 mM), NAA (1-napthaleneacetic acid; 0.54 mM ) and AgNO3 (5.89 mM). The excised microshoots were rooted efficiently on ½ MS medium supplemented with IBA (indole butyric acid; 9.8 mM) and BAP (0.11 mM). The rooted shoots were acclimatized in polybags containing garden soil, sand and FYM (1:1:1) and then transferred into the open. About 66.67% of the plants survived in the end. The genetic fidelity of the regenerated plantlets was found identical to the mother plant using SRAP primers. |
| Page(s): | 265-268 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.12(2) [April 2013] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 12(2) 265-268.pdf | 104.05 kB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.