Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/20949| Title: | A simple method for selective removal of human DNA from oral microbiome for functional metagenomic applications |
| Authors: | Hemalatha, Devaraj Rajendhran, Jeyaprakash Gunasekaran, Paramasamy |
| Keywords: | Filtration;Oral metagenome;Real time PCR;Quantification |
| Issue Date: | Sep-2013 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This study presents a simple filtration based technique to reduce human DNA contamination in mouthwash samples. Quantitative PCR evaluated efficiency of proposed method in removal of human DNA and selective extraction of bacterial DNA. The b-globin and 16S rDNA were targeted to measure the level of human and bacterial DNA respectively. When compared to unfiltered metagenomic DNA, there was 89% reduction in human DNA along with proportionate enrichment of bacterial DNA was obtained in filtered DNA. This strategy can serve as an efficient method for removal of human DNA and enrichment of microbial DNA in mouth wash samples usefull for functional metagenomic applications. |
| Page(s): | 572-576 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Appears in Collections: | JSIR Vol.72(09-10) [September-October 2013] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| JSIR 72(9-10) 572-576.pdf | 287.87 kB | Adobe PDF | View/Open |
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