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http://nopr.niscpr.res.in/handle/123456789/21849| Title: | Micropropagation of Stemona tuberosa Lour.—An endangered and rare medicinal plant in Eastern Ghats of India |
| Authors: | Murthy, K Sri Rama Reddy, M Chandrasekhara Kondamudi, R Pullaiah, T |
| Keywords: | Micropropagation;Multiple shoots;Stemona tuberosa;Tuberous roots |
| Issue Date: | Jul-2013 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | An in vitro technique of multiple plantlet regeneration was developed for conservation of endangered wild medicinal plant Stemona tuberosa Lour.. Murashige and Skoog (MS) medium supplemented with 7.0 mg/L Kn (kinetin) was found to be the optimum for axillary bud proliferation and multiple shoot induction. Excision and culture of nodal segments from the in vitro-shoots on medium containing 7.0 mg/L Kn and 4.0 mg/L TDZ (thidiazuron) showed maximum number of shoot multiplication with 7.10±0.37 shoots/node. Rapid shoot growth with simultaneous tuberous root formation was also observed in the same concentration. Shoots developed were rooted best on ½ strength MS with 1.0 mg/L IAA (indole acetic acid). Plantlets established in pots exhibited 85% survival. Plantlets successfully established in field exhibited morphological characters identical to mother plants. |
| Page(s): | 420-424 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.12(3) [July 2013] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 12(3) 420-424.pdf | 121.77 kB | Adobe PDF | View/Open |
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