Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/23431| Title: | Efficient regeneration of sorghum, Sorghum bicolor (L.) Moench, from shoot-tip explant |
| Authors: | Syamala, D Devi, Prathibha |
| Keywords: | Regeneration;Shoot-tip explants;Sorghum |
| Issue Date: | Dec-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Novel protocols for production of multiple shoot-tip clumps and somatic embryos of Sorghum bicolor (L.) Moench were developed with long-term goal of crop improvement through genetic transformation. Multiple shoot-tip clumps were developed in vitro from shoot-tip explant of one-week old seedling, cultured on MS medium containing only BA (0.5, 1 or 2 mg/l) or both BA (1 or 2 mg/I) and 2,4-D (0.5 mg/l) with bi-weekly subculture. Somatic embryos were directly produced on the enlarged dome shaped growing structures that developed from the shoot-tips of one-week old seedling explants (without any callus formation) when cultured on MS medium supplemented with both 2,4-D (0.5 mg/l) and BA (0.5 mg/I). However, the supplementation of MS medium with only 2, 4-D (0.5 mg/l) induced compact callus without any plantlet regeneration. Each multiple shoot-clump was capable of regenerating more than 80 shoots via an intensive differentiation of both axillary and adventitious shoot buds, the somatic embryos were capable of 90% germination, plant conversion and regeneration. The regenerated shoots could be efficiently rooted on MS medium containing indole-3-butyric acid (IBA 1 mg/l). The plants were successfully transplanted to glasshouse and grown to maturity with a survival rate of 98%. Morphogenetic response of the explants was found to be genotypically independent. |
| Page(s): | 1482-1486 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.41(12) [December 2003] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 41(12) 1482-1486.pdf | 1.23 MB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.