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http://nopr.niscpr.res.in/handle/123456789/24003| Title: | Effect of immunization with lipid associated polysaccharide antigen and anti CD-2 antibodies on class II MHC expression and cellular immune response in BALB/C mice infected with Leishmania donovani |
| Authors: | Bimal, Sanjiva Bagchi, A K Das, V Sinha, P K Lal, C S Ranjan, A Gupta, A K Kar, S K |
| Issue Date: | Sep-2001 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In a bid to characterize the antigens and immunization mechanisms which may be used to produce a protective response against L donovani, role of lipid associated polysaccharide (LPS) antigen and whole antigen was evaluated. BALB/C mice were immunized with whole or LPS antigen in combination with one of three putative adjuvents (anti CD-2 antibody I FIA I 0.85 % Saline). LPS antigen emulsified in anti CD-2 antibody was found to induce significant antibodies in mice on day 28 against challenge with lethal dose of L donovani. Immunoprophylactic properties of LPS and whole antigen was investigated on day 40 through cytokine elicitation (IL-2), MIF) in culture supernatants of spleen cells, but before that MHC-II expressed on macrophage was studied. The LPS antigen in combination with anti CD-2 antibody was found to be most immuno-reactive inducing higher MHC-II expression on macrophages which was associated with substantial rise in the level of MIF and IL-2. It coincided with decline in antibody titre in 100% mice immunized with LPS antigen while Leishmania injected as whole antigen failed to induce specific macrophage and T-cell response with all the above formulations. We surmise from our data that lipid associated polysaccharide antigen linked to anti CD-2 antibody has potential for eliciting protective immunity against Leishmania. |
| Page(s): | 878-882 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.39(09) [September 2001] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 39(9) 878-882.pdf | 1.45 MB | Adobe PDF | View/Open |
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