Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/24250| Title: | Somatic embryogenesis in Vigna radiata (L.) Wilczek |
| Authors: | Girija, S Ganapathi, A Ananthakrishnan, G |
| Issue Date: | Dec-2000 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Somatic embryogenesis was achieved from immature cotyledon derived callus of mungbean, V.radiata (L.) Wilczek in MS liquid medium. Embryogenic callus was induced on MS medium with NAA (5mg/L). Differentiation of somatic embryos was observed when embryogenic callus was transferred to MS liquid medium containing 2, 4-D (1.5 mg/L) and L-proline (50 mg/L). The torpedo shaped embryos were transferred to MS liquid medium with BAP and ABA (1 mg/L each) for maturation and germination. Fifty per cent of torpedo shaped embryos were converted into tiny plants (8-9 plants out of 17) after one week of culture. The germinated embryos were isolated and transferred to MS half strength basal (solid) medium for further development. |
| Page(s): | 1241-1244 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.38(12) [December 2000] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 38(12) 1241-1244.pdf | 811.73 kB | Adobe PDF | View/Open |
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