Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/27727| Title: | Submerged fermentation and characterization of carboxymethyl cellulase from a rhizospheric isolate of Trichoderma viride associated with Azadirachta indica |
| Authors: | Bhattacharya, Sourav Das, Arijit Patnaik, Amie Bokade, Prianka Rajan, S. Sundara |
| Keywords: | Trichoderma viride;Carboxymethyl cellulase;Submerged fermentation;Media optimization |
| Issue Date: | Apr-2014 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Analysis of process parameters influencing the submerged fermentation of carboxymethyl cellulase by Trichoderma viride isolated from Azadirachta indica rhizosphere showed that the highest enzyme yield was obtained with carboxymethyl cellulose and ammonium sulphate as nutritional supplements at an optimum pH, temperature, incubation period and inoculum volume of 5.0, 30°C, 72 h and 1% (v/v), respectively. Sodium dodecyl sulphate polyacrylamide gel electrophoresis revealed the apparent molecular weight of the purified enzyme to be 55 kDa. The enzyme exhibited a Vmax value of 83.7 µg/min and Km value of 0.53 mg/ml. Optimal activity of the enzyme at 50°C and pH 5.0 emphasizes its potential to be utilized in textile industry operations. |
| Page(s): | 225-230 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Appears in Collections: | JSIR Vol.73(04) [April 2014] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| JSIR 73(4) 225-230.pdf | 145.65 kB | Adobe PDF | View/Open |
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