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http://nopr.niscpr.res.in/handle/123456789/28713| Title: | Optimization of microwave pretreatment and enzymatic hydrolysis of pith bagasse with Trichoderma cellulase |
| Authors: | Farid, M A El-Deen, A M Noor Shata, H M |
| Keywords: | Box-Behnken design;Hydrolysis;Microwave treatment;Optimization;Pith bagasse;Trichoderma cellulase |
| Issue Date: | Jan-2014 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Pith bagasse was subjected to microwave/chemical pretreatments at 900 W under atmospheric pressure for a short time (2-6 min) through several cycles (30 sec/cycle). The pith bagasse lost about 48, 21 and 40% of its wt after treatment with microwave/NaOH, microwave/H2SO4 and microwave/H2O2/acetic acid, respectively. Enzymatic hydrolysis of pretreated substrates with Trichoderma cellulase indicated that pith bagasse pretreated with microwave/H2O2/acetic acid and microwave/NaOH had the highest hydrolysis rate and sugar yield. The mean value for the total reducing sugars (TRS) and glucose yields from both treatments are approx 4 to 5 times than microwave/H2SO4 pretreated sample. Optimization of treatment process using Box-Behnken design at 6 and 24 h showed that the linear and quadratic terms of the 3 variables tested [H2O2/acetic acid treatment time (X1), solid:liquid ratio (X2) and microwave treatment time (X3)] had significant effects. Maximal concentrations of TRS and glucose (22 and 18.33 mg/mL, respectively) could be obtained after 24 h hydrolysis time when X1, X2 and X3 were set at 60 min, 1:20 (w/v) and 4 min, respectively. The yield of TRS reached about 99% (g/g total dry matter). |
| Page(s): | 98-107 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.13(1) [January 2014] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 13(1) 98-107.pdf | 271.85 kB | Adobe PDF | View/Open |
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