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http://nopr.niscpr.res.in/handle/123456789/29151| Title: | Direct and callus mediated regeneration from nodal and internodal segments of Crataeva religiosa G. Forst. var. nurvala (Buch.-Ham.) Hook. f. & Thomson |
| Authors: | Prakash, Anand Kumari, Sanju Utkarshini Sinha, Kanak Kumar, Santosh |
| Keywords: | Callus;Crataeva religiosa var. nurvala;In vitro regeneration;Shoot multiplication |
| Issue Date: | Apr-2014 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Crataeva religiosa G. Forst. var. nurvala (Buch.-Ham.) Hook. F. & Thomson (syn. Crataeva nurvala Buch.-Ham.), a fast growing medicinal tree species, has been successfully micropropagated under in vitro conditions. Direct multiple shoots were regenerated from nodal explants on Murashige and Skoog’s (MS) medium, solidified by agar and supplemented with 1 mg/L 6-benzyl amino purine (BAP) and 0.5 mg/L α-naphthalene acetic acid (NAA). However, the successful regeneration from internodal callus was achieved on 2.0 mg/L BAP and 0.5 mg/L NAA. The frequency of in vitro multiplication from culture of nodal segments was higher compared to that of callus mediated regeneration from culture of internodal segments. Further, the best rhizogenesis was achieved on solidified MS medium supplemented with 3 mg/L indole-3-butyric acid (IBA). |
| Page(s): | 263-267 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.13(2) [April 2014] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 13(2) 263-267.pdf | 285.77 kB | Adobe PDF | View/Open |
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