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http://nopr.niscpr.res.in/handle/123456789/31441| Title: | Production of 4-Ipomeanol, an anticancer agent from the root tubers and rhizogenic callus of Ipomoea batatas Lam.: A Comparative study |
| Authors: | Mohanraj, Remya Subha, S |
| Keywords: | Callus culture;Chitin;Chitosan;Furanoterpenoid;Sweet potato;Tumors |
| Issue Date: | May-2015 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A comparative study was done on the production of 4-ipomeanol from root tubers of Ipomoea batatas and its rhizogenic callus. Best callusing response was obtained on MS medium supplemented with 11 µM NAA (α-Naphthaleneacetic acid) and 1 µM KIN (Kinetin). Effect of various elicitors (Fusarium solani, chitin and chitosan) on the production of 4-ipomeanol was studied. Methanol extract of the samples were purified by column chromatography and detected using TLC. Identification of 4-ipomeanol was confirmed using HPLC and quantified spectrophotometrically. A mass spectrum was recorded to confirm the presence of 4-ipomeanol. The calli grown under chitin produced highest (6.61mg g-1) amount of 4-ipomeanol. This is the first report on in vitro production of 4-ipomeanol from I. batatas. Since 4-ipomeanol is reported to be present only in I. batatas, this study would help in standardizing protocols for large scale production without affecting its natural flora. |
| Page(s): | 297-304 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.53(05) [May 2015] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 53(5) 297-304.pdf | 258.35 kB | Adobe PDF | View/Open |
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