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http://nopr.niscpr.res.in/handle/123456789/31909| Title: | Small concentrations of TGF-β1 promote proliferation of bone marrow-derived mesenchymal stem cells via activation of Wnt/β-catenin pathway |
| Authors: | Zhang, Fenxi Ren, Tongming Wu, Junfang Niu, Jie |
| Keywords: | BMSC proliferation;p53 expression;Stem cells;Transforming growth factor-β1 |
| Issue Date: | Aug-2015 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Bone marrow-derived mesenchymal stem cells (BMSCs) are the most promising seed cells for cell transplant. The proliferation of BMSCs is one of the most important determinants of the efficiency of MSC-based transplant therapy. It has been reported that transforming growth factor-β1 (TGF-β1) activates Wnt/β-catenin signaling and regulates cell proliferation. In this study, we investigated the effect of low concentrations of TGF-β1 on proliferation of BMSCs and the related mechanisms. BMSCs were treated with 0, 1, 5 and 10 ng/L recombinant mouse TGF-β1 for 12 h. Cell proliferation was measured by cell counting and MTT assay, and the proliferation-related signals p53, Mdm2, Akt1, Wnt3, phospho-Akt and β-catenin were measured by quantitative polymerase chain reaction (qPCR) and/or Western blot. Our results showed that TGF-β1 at low concentrations induced BMSC proliferation and expression of Mdm2, Akt1, phospho-Akt, Wnt3 and β-catenin, and inhibited p53 expression in dose dependent manner. Importantly, β-catenin siRNA significantly inhibited TGF-β1-induced BMSC proliferation. These findings suggest that low concentrations of TGF-β1 can stimulate proliferation of BMSCs, which is at least partially dependent on the activation of Wnt/β-catenin pathway. |
| Page(s): | 508-513 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.53(08) [August 2015] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 53(8) 508-513.pdf | 274.1 kB | Adobe PDF | View/Open |
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