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http://nopr.niscpr.res.in/handle/123456789/33414| Title: | MPGIT: A concentrated monophasic phenol-guanidinium isothiocyanate reagent for isolation of viral RNA |
| Authors: | Gauthami, S Meena, Keerti Kumar, Deepak Uma, Madala Reddy, Y Vishnuvardhan Rao, Pavuluri Panduranga Hegde, Nagendra R |
| Keywords: | Acid phenol;RNA isolation;RT-PCR;Virus R NA |
| Issue Date: | Jul-2015 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A method to prepare a concentrated monophasic phenol guanidinium isothiocyanate-based reagent for extraction of RNA from liquid samples is described. The reagent was comparable to a commercial reagent for extraction of genetic material of several RNA viruses as assessed by limit of detection of RNA by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The study showed that the concentrated monophasic reagent could be used in place of a commercial reagent. |
| Page(s): | 376-381 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.14(3) [July 2015] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 14(3) 376-381.pdf | 165.68 kB | Adobe PDF | View/Open |
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