<span style="font-size:15.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Malgun Gothic";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Simultaneous production of biodiesel and bioethanol through mixotrophic cultivation of <i style="mso-bidi-font-style:normal">Chlorella</i> sp.</span>

Abstract

<span style="font-size:9.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"malgun="" gothic";mso-bidi-font-family:="" "times="" roman";mso-ansi-language:en-gb;mso-fareast-language:en-us;="" mso-bidi-language:hi"="" lang="EN-GB">It is a comparative study on the effects of cultivation conditions in the simultaneous production of biodiesel and ethanol using microalga <i style="mso-bidi-font-style:normal">Chlorella </i>sp. Intracellular lipid and reducing sugar were extracted from the biomass using the solvent extraction to conduct comparison of the yield, fatty acids profile configuration, and monosaccharide composition. The 27-days cultivation of Chlorella sp. in 20 ℓ photobioreactor under mixo-, hetero-, and autotrophic conditions presented the cell concentration, lipid and reducing sugar yield at 4.13, 2.10, 3.50 g•L^-1, 24.1, 18.4, 16.7 (%, w/w), and 30.6, 27.5, 29.1 (%, w/w), respectively. Result shows that <i style="mso-bidi-font-style:normal">Chlorella</i> sp. achieves the highest lipid and reducing sugar yield under the mixotrophic condition with 0.0369 and 0.0468 g•L^-1•d^-1, respectively. Also, <i style="mso-bidi-font-style:normal">Chlorella</i> sp. when cultivated using the mixotrophic condition, builds better fatty acid and reducing sugar in the cell that can be synthesized for biodiesel and ethanol production.</span>