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http://nopr.niscpr.res.in/handle/123456789/43627| Title: | Urease immobilized potentiometric biosensor for determination of urea |
| Authors: | Sihombing, Kawan Tamba, Monica Chrisdayanti Marbun, Winry Sulistiani Situmorang, Manihar |
| Keywords: | Electroanalytical chemistry;Biosensors;Potentiometric biosensors;Enzyme electrode;Tungsten electrode;Immobilized enzyme;Urea |
| Issue Date: | Feb-2018 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A simple potentiometric biosensor for the determination of urea is reported in this work. The biosensor has been constructed by immobilization of urease at tungsten electrode using polyvinyl alcohol matrix followed by the sealing process with plasticised polyvinyl chloride. The sensing device gives fast and sensitive response to urea. A linear calibration curve in the concentration range of 0.01–50 mM urea is obtained with slope 42.7 mV per decade urea concentration (r2 = 0.993), and detection a limit of 0.01 mM urea. |
| Page(s): | 175-180 |
| ISSN: | 0975-0975(Online); 0376-4710(Print) |
| Appears in Collections: | IJC-A Vol.57A(02) [February 2018] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJCA 57A(2) 175-180.pdf | Main Article | 213.24 kB | Adobe PDF | View/Open |
| IJCA 57A(2) 175-180_Suppl Data.pdf | Supplementary Data | 89.41 kB | Adobe PDF | View/Open |
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